منابع مشابه
Diagnosis of complete molar pregnancy by microsatellites in archival material.
AIMS To develop an assay which would determine the parentage of hydatidiform molar pregnancies. METHODS DNA was extracted from formalin fixed, paraffin wax embedded tissue from hydatidiform molar pregnancies and spontaneous abortions after separation of chorionic villi and decidua. PCR amplification of dinucleotide repeat sequences ("microsatellites") was performed using three different prime...
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The extraction and amplification of nucleic acid from formalin-fixed and paraffin-embedded tissues has become an important exercise in the collection of retrospective epidemiological data. A protocol is described that enables the extraction and amplification of dsDNA from fixed tissues within paraffin blocks and from specimens stored in 10% (aq) formalin. The procedure can be used for the exami...
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World War II was a global conflict that dramatically changed societies and landscapes around the world. It also left behind a vast and diverse material record. Although the battles of the conflict never reached Fiji, a British colony in the South Pacific, the War's impact on the island group is clear. Through 1942 and part of 1943, while under threat of Japanese invasion, defensive installation...
متن کاملStored human tissue: an ethical perspective on the fate of anonymous, archival material.
The furore over the retention of organs at postmortem examination, without adequate consent, has led to a reassessment of the justification for, and circumstances surrounding, the retention of any human material after postmortem examinations and operations. This brings into focus the large amount of human material stored in various archives and museums, much of which is not identifiable and was...
متن کاملGene Expression, Single Nucleotide Variant and Fusion Transcript Discovery in Archival Material from Breast Tumors
Advantages of RNA-Seq over array based platforms are quantitative gene expression and discovery of expressed single nucleotide variants (eSNVs) and fusion transcripts from a single platform, but the sensitivity for each of these characteristics is unknown. We measured gene expression in a set of manually degraded RNAs, nine pairs of matched fresh-frozen, and FFPE RNA isolated from breast tumor ...
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ژورنال
عنوان ژورنال: Aboriginal History Journal
سال: 2011
ISSN: 0314-8769,1837-9389
DOI: 10.22459/ah.16.2011.02